NIH funded ME/CFS Collaborative Research Center (U54NS105541) Single Cell Transcriptomics Sequencing of mRNA and microRNA from peripheral blood to identify specific dysregulated leukocytes. Subject Participation Volunteers will be able to participate in exercise tests and sample collection at three locations. Vesicles and Signaling Examining the effect of exercise on extracellular vesicle cargo, inflammatory proteins, and metabolism. Data Analysis Skilled analysts will support projects with bioinformatics, data management and data integration. Oxidative Stress and Neuroinflammation Using MRI and PET imaging to measure levels of energy-related and inflammatory molecules in the central nervous system. Other ENID Center Projects Genomics Examining the expression of genes in immune cells and mitochondrial genomes. Metabolism Probing the utilization of mitochondrial oxidative phosphorylation vs. glycolysis in immune cells and accumulation of metabolites in plasma. Microbiome Comparing gut microbiota, including bacteria, eukaryotes, and viruses in ME/CFS patients and controls. Brain Function Using state-of-the-art neuroimaging to study oxidative stress in ME/CFS.