Endothelial Function and Hemostasis


Image:
Cultured human brain microvascular endothelial cells
Cell Systems, Inc.

Increasing evidence implicates disturbances in hemostasis and endothelial function in ME/CFS. Assays for endothelial dysfunction can be performed by measurements of circulation by the EndoPat® as well as by examining endothelial cells in vitro and in vivo. Gene expression in endothelial cells in muscle biopsies from ME/CFS subjects and controls will be compared. A recent report indicated that cultured endothelial cells treated with plasma from ME/CFS subjects caused less nitric oxide (NO) production than when cells were treated with control plasma. We will attempt to repeat and extend these observations.


Image:
Human brain microvascular endothelial cells
Cell Systems, Inc.

Ghebremariam et al. 2014. J Vasc Res. 51(1): 68–79.
Fluorescent microscopy reveals cells producing nitric oxide

Members of the Hanson lab and Grimson lab will collaborate to assay endothelial function in vitro. Gene expression in endothelial cells will be examined through spatial transcriptomics and single nucleus RNAseq in the Cosgrove and de Vlaminck labs. Data will also be provided through the measurements performed at the Hospital for Special Surgery.

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