NIH ME/CFS Collaborative Research Center 2017-2023 (U54NS105541) Single Cell Transcriptomics Sequencing of mRNA and microRNA from peripheral blood to identify specific dysregulated leukocytes. Two-Day Cardiopulmonary Exercise Testing Volunteers participated in exercise testing and sample collection at three locations. Vesicles and Signaling Examining the effect of exercise on extracellular vesicle cargo, inflammatory proteins, and metabolism. Data Analysis Skilled analysts will support projects with bioinformatics, data management and data integration. Oxidative Stress and Neuroinflammation Using MRI and PET imaging to measure levels of energy-related and inflammatory molecules in the central nervous system.